2IPP

Crystal Structure of the tetragonal form of human liver cathepsin B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	271
Detector technology	AREA DETECTOR
Collection date	1989-07-31
Detector	ADSC
Wavelength(s)	1.5418
Spacegroup name	P 43
Unit cell lengths	85.580, 85.580, 34.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.150
R-factor	0.157
Structure solution method	MOLECULAR REPLACEMENT
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	19.500
High resolution limit [Å]	2.000
Rmerge	0.102
Number of reflections	13463
Completeness [%]	99.3
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K