2IPJ

Crystal structure of h3alpha-hydroxysteroid dehydrogenase type 3 mutant Y24A in complex with NADP+ and epi-testosterone

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (1.8 Å)

Cell axes144.788144.788203.885
Cell angles90.0090.00120.00
SpacegroupH 3 2
Resolution limits19.98 - 1.80
the highest resolution shell value1.810 - 1.800
R-work0.20400
the highest resolution shell value0.315
R-free0.22800
the highest resolution shell value0.338
RMSD bond length0.012
RMSD bond angle1.300

Data Collection Statistics

Resolution limits19.98 - 1.80
the highest resolution shell value -
Number of reflections75736
Rmerge_l_obs0.068
the highest resolution shell value0.278
Completeness99.7
Redundancy7.4
the highest resolution shell value7.4
I/sigma(I)0

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1hanging drop5.6277
1hanging drop5.6277
1hanging drop5.6277

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*