2IPB
Crystal structure of T159D mutant of S. Typhimurium PhoN protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200HB |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.273, 45.113, 112.945 |
| Unit cell angles | 90.00, 112.05, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.230 |
| R-factor | 0.154 |
| Rwork | 0.151 |
| R-free | 0.20800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2a96 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.929 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.330 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.079 | 0.182 |
| Number of reflections | 43054 | |
| <I/σ(I)> | 12.7 | 4.7 |
| Completeness [%] | 95.6 | 75.8 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% PEG-6000, 25mM potassium chloride, 5mM magnesium sulphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
