2IP2
Structure of the Pyocyanin Biosynthetic Protein PhzM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-08-04 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.541 |
Spacegroup name | P 1 |
Unit cell lengths | 46.970, 62.410, 68.750 |
Unit cell angles | 97.47, 105.37, 108.09 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.1963 |
Rwork | 0.194 |
R-free | 0.24739 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.744 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | RESOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.400 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.110 | 0.371 |
Number of reflections | 60747 | |
<I/σ(I)> | 11.3 | 2.6 |
Completeness [%] | 94.2 | 90.7 |
Redundancy | 7.36 | 7.31 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | The well solution contained 18-21% PEG 3350, 0.1M magnesium chloride, 0.1M MOPS, pH 7.0. The drops were formed by mixing equal volumes of protein (13.5 mg/ml) and well solution, VAPOR DIFFUSION, SITTING DROP, temperature 293K |