2IOS
Crystal structure of the C-terminal MA3 domain of Pdcd4 (mouse); form 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 68.254, 64.021, 38.453 |
| Unit cell angles | 90.00, 114.15, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.760 |
| R-factor | 0.19718 |
| Rwork | 0.195 |
| R-free | 0.24853 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iol chain A. |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.792 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.806 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 14854 | |
| <I/σ(I)> | 32.2 | 4.54 |
| Completeness [%] | 99.5 | 95.6 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 10% (w/v) PEG-3000, 100 mM phosphate-citrate, 200 mM NaCl, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
