2IOQ
Crystal Structure of full-length HTPG, the Escherichia coli HSP90
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.08 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 105.124, 105.124, 531.077 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.700 - 3.500 |
Rwork | 0.329 |
R-free | 0.37000 |
Structure solution method | MIR |
RMSD bond length | 0.002 |
RMSD bond angle | 0.684 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.700 | 3.630 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 22816 | |
<I/σ(I)> | 21.4 | 2.6 |
Completeness [%] | 98.4 | 98.2 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 5-10% PEG3000 or PEG4000, 30-60 mM MgCl2, 50 mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |