2IOH
Crystal structure of phosphonoacetaldehyde hydrolase with a K53R mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-03 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.50000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 251.728, 251.728, 156.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| Rwork | 0.253 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.511 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.120 |
| Number of reflections | 50000 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Ammonium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
