2IO9

E. coli Bifunctional glutathionylspermidine synthetase/amidase Incomplex with Mg2+ ,GSH and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2005-03-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	59.912, 75.480, 84.006
Unit cell angles	70.44, 74.17, 78.06

Refinement procedure

Resolution	30.000 - 2.200
Rwork	0.170
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.372
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200
Rmerge	0.075
Number of reflections	64297
<I/σ(I)>	26
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	298	12% PEG 3350, 0.5M magnesium chloride, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, temperature 298K