2INV
Crystal structure of Inulin fructotransferase in the presence of di-fructose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-16 |
| Detector | BRUKER PROTEUM 300 |
| Wavelength(s) | 1.12714 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.166, 91.900, 92.916 |
| Unit cell angles | 90.00, 124.82, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| Rwork | 0.207 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2inu |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.286 |
| Number of reflections | 93099 | |
| <I/σ(I)> | 29.6 | 5 |
| Completeness [%] | 91.9 | 100 |
| Redundancy | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 1.0M ammonium phosphate, 0.1M imidazole, 0.2M sodium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
