2INS
THE STRUCTURE OF DES-PHE B1 BOVINE INSULIN
Experimental procedure
| Spacegroup name | H 3 |
| Unit cell lengths | 81.600, 81.600, 34.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.500 |
| R-factor | 0.18 * |
| Rwork | 0.180 |
| RMSD bond length | 0.020 * |
| RMSD bond angle | 3.000 |
| Refinement software | FAST (FOURIER LEAST-SQUARES REFINEMENT) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 * |
| Number of reflections | 2722 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 5.5mg | |
| 2 | 1 | 1 | 0.02 (M) | 1.0ml | |
| 3 | 1 | 1 | zinc acetate | 0.15 (M) | 0.1ml |
| 4 | 1 | 1 | sodium citrate | 0.2 (M) | 0.5ml |
| 5 | 1 | 1 | acetone | 0.3ml |
