2IN5
Crystal Structure of the hypothetical lipoprotein YmcC from Escherichia coli (K12), Northeast Structural Genomics target ER552.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.793, 74.998, 80.201 |
| Unit cell angles | 90.00, 127.11, 90.00 |
Refinement procedure
| Resolution | 37.500 - 2.300 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.075 | 0.320 |
| Number of reflections | 50365 | |
| <I/σ(I)> | 19.1 | 4.2 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M Tris, 3.0M Sodium Chloride, 5mM DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
