2IMZ

Crystal structure of Mtu recA intein splicing domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-01-01
Detector	BRANDEIS - B4
Wavelength(s)	1.011, 1.091, 0.94
Spacegroup name	P 21 21 2
Unit cell lengths	82.430, 93.630, 40.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.760 - 1.700
R-factor	0.181
Rwork	0.181
R-free	0.21000
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.750
High resolution limit [Å]	1.700	1.700
Rmerge	0.043	0.207
Number of reflections	40459
<I/σ(I)>	27.1	4.6
Completeness [%]	89.4	92.1
Redundancy	4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	283	6% PEG8000, 0.1 M Tris.HCl, 5% PEG400, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K
1	VAPOR DIFFUSION, HANGING DROP	8.5	283	6% PEG8000, 0.1 M Tris.HCl, 5% PEG400, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K