2IMT
The X-ray Structure of a Bak Homodimer Reveals an Inhibitory Zinc Binding Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-06-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 57.247, 53.646, 58.074 |
Unit cell angles | 90.00, 114.87, 90.00 |
Refinement procedure
Resolution | 37.320 - 1.490 |
R-factor | 0.19393 |
Rwork | 0.193 |
R-free | 0.22010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ims |
RMSD bond length | 0.027 |
RMSD bond angle | 1.950 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.540 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.041 | 0.271 |
Number of reflections | 21001 | |
<I/σ(I)> | 14.7 | 2.7 |
Redundancy | 3.34 | 1.83 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG 3350 15-30% and 0.2 M ammonium fluoride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |