2IMT
The X-ray Structure of a Bak Homodimer Reveals an Inhibitory Zinc Binding Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.247, 53.646, 58.074 |
| Unit cell angles | 90.00, 114.87, 90.00 |
Refinement procedure
| Resolution | 37.320 - 1.490 |
| R-factor | 0.19393 |
| Rwork | 0.193 |
| R-free | 0.22010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ims |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.950 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.041 | 0.271 |
| Number of reflections | 21001 | |
| <I/σ(I)> | 14.7 | 2.7 |
| Redundancy | 3.34 | 1.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG 3350 15-30% and 0.2 M ammonium fluoride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
