2IMS
The X-ray Structure of a Bak Homodimer Reveals an Inhibitory Zinc Binding Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.514 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.527, 53.662, 58.281 |
| Unit cell angles | 90.00, 115.05, 90.00 |
Refinement procedure
| Resolution | 52.700 - 1.480 |
| R-factor | 0.1805 |
| Rwork | 0.179 |
| R-free | 0.20402 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.437 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.520 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.040 | 0.487 |
| Number of reflections | 27038 | |
| <I/σ(I)> | 36.5 | 2.17 |
| Completeness [%] | 98.6 | 86.7 |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG 3350 15-30% and 1-50 mM zinc acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
