2IMQ
Crystal structure of ferrous cimex nitrophorin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.107, 41.491, 65.380 |
| Unit cell angles | 90.00, 95.40, 90.00 |
Refinement procedure
| Resolution | 28.350 - 1.300 |
| R-factor | 0.17753 |
| Rwork | 0.176 |
| R-free | 0.20844 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ntf |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.754 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.400 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.067 | 0.330 |
| Number of reflections | 64243 | |
| <I/σ(I)> | 17.6 | 2.4 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 7.8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 23% PEG 4000, 100 mM Tris HCl, 200 mM lithium sulfate, 15% ethanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
