2IMH
Crystal structure of protein SPO2555 from Silicibacter pomeroyi, Pfam DUF1028
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-09-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.250, 69.594, 105.792 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.570 |
| R-factor | 0.17253 |
| Rwork | 0.171 |
| R-free | 0.20660 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.298 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.093 | 0.440 |
| Number of reflections | 51467 | |
| <I/σ(I)> | 15.4 | 1.4 |
| Completeness [%] | 82.7 | 27.4 |
| Redundancy | 7.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 560mM tri-Sodium Citrate dihydrate, pH 7, VAPOR DIFFUSION, temperature 294K |
