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2IM8

X-Ray Crystal Structure of Protein yppE from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR213.

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X4A
Synchrotron siteNSLS
BeamlineX4A
Temperature [K]100
Detector technologyCCD
Collection date2006-08-24
DetectorADSC QUANTUM 4
Wavelength(s)0.97934, 0.97956, 0.96806
Spacegroup nameP 21 21 21
Unit cell lengths44.735, 70.830, 76.237
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution19.290 - 2.000
R-factor0.236
Rwork0.236
R-free0.25700
Structure solution methodMAD
RMSD bond length0.006
RMSD bond angle1.100
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareSOLVE
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.070
High resolution limit [Å]2.0002.000
Rmerge0.0750.334
Number of reflections30962
<I/σ(I)>29.27.5
Completeness [%]97.793.4
Redundancy33.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.52930.8M KH2PO4, 0.8M NaH2PO4, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

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