2IM8
X-Ray Crystal Structure of Protein yppE from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR213.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97934, 0.97956, 0.96806 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.735, 70.830, 76.237 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.290 - 2.000 |
R-factor | 0.236 |
Rwork | 0.236 |
R-free | 0.25700 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.075 | 0.334 |
Number of reflections | 30962 | |
<I/σ(I)> | 29.2 | 7.5 |
Completeness [%] | 97.7 | 93.4 |
Redundancy | 33.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.8M KH2PO4, 0.8M NaH2PO4, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |