2ILM
Factor Inhibiting HIF-1 Alpha D201A Mutant in Complex with FE(II), Alpha-Ketoglutarate and HIF-1 Alpha 35mer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95372 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.531, 86.531, 147.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.270 - 2.300 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.26700 |
Structure solution method | ISOMORPHOUS WITH 1H2N |
Starting model (for MR) | 1h2n |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.270 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.067 | 0.465 |
Number of reflections | 25664 | |
<I/σ(I)> | 21.2 | 2.18 |
Completeness [%] | 99.8 | 97.1 |
Redundancy | 11.4 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 1.6M AMMONIUM SULPHATE, 6% PEG400, 0.1M HEPES PH7.5 ARGON ATMOSPHERE, 14MG/ML PROTEIN WITH 1MM FE(II)SO4, 1MM ALPHA-KETOGLUTARATE, 1MM HIF-1ALPHA PEPTIDE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298.0K, pH 7.50 |