2ILK
CRYSTAL STRUCTURE OF HUMAN INTERLEUKIN-10 AT 1.6 ANGSTROMS RESOLUTION
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05-04 |
| Detector | FUJI |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.530, 69.530, 70.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.163 * |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.048 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.072 * |
| Number of reflections | 27529 |
| <I/σ(I)> | 1 |
| Completeness [%] | 86.4 |
| Redundancy | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 10 (%) | |
| 3 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 60 (%sat) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
