2IL5
Structure of Protein of Unknown Function SA2116 from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-13 |
| Detector | SBC |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 62 |
| Unit cell lengths | 107.588, 107.588, 39.105 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.200 - 2.300 |
| R-factor | 0.2057 |
| Rwork | 0.202 |
| R-free | 0.24520 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.664 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.200 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.102 | 0.797 |
| Number of reflections | 11546 | |
| <I/σ(I)> | 49.2 | 3 |
| Completeness [%] | 98.4 | 88.4 |
| Redundancy | 20.4 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.2 M Proline, 0.1 M HEPES buffer, 10% PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
