2IKS
Crystal structure of N-terminal truncated DNA-binding transcriptional dual regulator from Escherichia coli K12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97907 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.931, 41.829, 110.674 |
| Unit cell angles | 90.00, 126.18, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.18199 |
| Rwork | 0.180 |
| R-free | 0.21949 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.482 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.075 | 0.415 |
| Number of reflections | 48758 | |
| <I/σ(I)> | 40.9 | 4.47 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 7.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 298 | 0.3m MgChloride,0.1M Bicine pH8.9, 20% PEG 2K, 1mM fructose 1,6-biphosphate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
