2IKF

Terminal uridylyl transferase 4 from Trypanosoma brucei with bound UTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	78.773, 41.525, 103.792
Unit cell angles	90.00, 94.94, 90.00

Refinement procedure

Resolution	51.709 - 2.000
Rwork	0.228
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b51
RMSD bond length	0.014
RMSD bond angle	1.777
Data reduction software	d*TREK
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	103.407	51.710	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge	0.098	0.027	0.573
Total number of observations		4272	18455
Number of reflections	43289
<I/σ(I)>	6.3	23.5	0.8
Completeness [%]	94.5	84.6	95.6
Redundancy	3	3.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	100 mM sodium cacodylate pH 6.5, 200 mM calcium acetate, 18% PEG-8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K