2IKB
Crystal Structure of a Protein of Unknown Function NMB1012 from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.335, 52.087, 128.874 |
| Unit cell angles | 90.00, 90.68, 90.00 |
Refinement procedure
| Resolution | 38.380 - 1.700 |
| R-factor | 0.19717 |
| Rwork | 0.195 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.410 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.744 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.105 | 0.371 |
| Number of reflections | 64965 | |
| <I/σ(I)> | 15.3 | 2.47 |
| Completeness [%] | 98.6 | 93.61 |
| Redundancy | 4.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 20% w/v PEG MME2000, 0.1M Tris-HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
