2IKB
Crystal Structure of a Protein of Unknown Function NMB1012 from Neisseria meningitidis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97980 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.335, 52.087, 128.874 |
Unit cell angles | 90.00, 90.68, 90.00 |
Refinement procedure
Resolution | 38.380 - 1.700 |
R-factor | 0.19717 |
Rwork | 0.195 |
R-free | 0.24700 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.410 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.744 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.105 | 0.371 |
Number of reflections | 64965 | |
<I/σ(I)> | 15.3 | 2.47 |
Completeness [%] | 98.6 | 93.61 |
Redundancy | 4.8 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 20% w/v PEG MME2000, 0.1M Tris-HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |