2IJR
Crystal structure of a protein api92 from Yersinia pseudotuberculosis, Pfam DUF1281
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .9792 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 88.656, 88.656, 218.227 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.22747 |
| Rwork | 0.225 |
| R-free | 0.27917 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.670 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.134 | 0.293 |
| Number of reflections | 11612 | |
| <I/σ(I)> | 9.6 | 2.7 |
| Completeness [%] | 57.0 | 51.9 |
| Redundancy | 3.8 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.5M Magnesium formate, 0.1M Bis Tris, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
