2IJI
Structure of F14H mutant of ColE1 Rom protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 39.140, 39.140, 57.442 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.100 - 2.300 |
| R-factor | 0.23675 |
| Rwork | 0.234 |
| R-free | 0.29163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rop |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.345 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER (1.2) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.100 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 0.072 |
| Number of reflections | 2378 | |
| <I/σ(I)> | 11.2 | 2.3 |
| Completeness [%] | 96.8 | 98.7 |
| Redundancy | 3.44 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 1uL protein solution (0.5-1 mM) was mixed with 1 uL well buffer (0.1M sodium acetate pH 5.5, 0.1M sodium chloride, 37 40% ethanol, and 20% glycerol), VAPOR DIFFUSION, HANGING DROP |
