2IJE

Crystal Structure of the Cdc25 domain of RasGRF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Detector technology	CCD
Collection date	2005-05-20
Spacegroup name	C 2 2 21
Unit cell lengths	75.430, 77.801, 110.303
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.205
Rwork	0.200
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nvv
RMSD bond length	0.008
RMSD bond angle	1.195
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.089	0.541
Number of reflections	17115
<I/σ(I)>	17.9	3.6
Completeness [%]	99.9	99.8
Redundancy	6.2	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	277	1:1 Mixture of Well Solution: 0.1 M Sodium Acetate, pH 5.2, 3% PEG 4000, 0.5% beta-octylglucoside. Protein Stock: 10 mg/ml RasGRF1, 10% Glycerol, 5 mM TCEP, 25 mM Tris, pH 8.0, 200 mM Sodium Chloride. Cryoprotection: Dip in Well solution with 30% sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 277K