2IJ3
Structure of the A264H mutant of cytochrome P450 BM3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-18 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.703, 155.173, 62.198 |
| Unit cell angles | 90.00, 94.19, 90.00 |
Refinement procedure
| Resolution | 19.980 - 1.900 |
| R-factor | 0.17887 |
| Rwork | 0.177 |
| R-free | 0.21821 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ij2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.124 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.071 |
| Number of reflections | 81622 |
| <I/σ(I)> | 11.1 |
| Completeness [%] | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | PEG and Magnesium sulphate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
