2IJ0

Structural basis of T cell specificity and activation by the bacterial superantigen toxic shock syndrome toxin-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Wavelength(s)	0.978
Spacegroup name	C 1 2 1
Unit cell lengths	190.898, 68.372, 53.549
Unit cell angles	90.00, 106.24, 90.00

Refinement procedure

Resolution	25.000 - 2.250
Rwork	0.242
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.250
Number of reflections	32180
<I/σ(I)>	21.5
Completeness [%]	99.4
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1M Sodium Cacodylate, 0.2 M Magnesium Acetate, PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K