2IIK
Crystal Structure of human peroxisomal acetyl-CoA acyl transferase 1 (ACAA1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-07-27 |
Detector | RIGAKU RAXIS HTC |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.147, 96.845, 142.055 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.060 - 2.550 |
R-factor | 0.18108 |
Rwork | 0.179 |
R-free | 0.22345 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c7z |
RMSD bond length | 0.013 |
RMSD bond angle | 1.402 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.060 | 2.650 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.110 | 0.401 |
Number of reflections | 25358 | |
<I/σ(I)> | 9.65 | 2.17 |
Completeness [%] | 99.0 | 96.2 |
Redundancy | 3.66 | 2.79 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2M Amonium Sulphate, 0.1M Hepes, 25% PEG3350., pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |