2IID
Structure of L-amino acid oxidase from Calloselasma rhodostoma in complex with L-phenylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Wavelength(s) | 0.652549 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.760, 154.003, 103.183 |
| Unit cell angles | 90.00, 109.52, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.17354 |
| Rwork | 0.172 |
| R-free | 0.21006 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1f8r |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.489 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.101 | 0.418 |
| Number of reflections | 211556 | |
| <I/σ(I)> | 11.9 | 3.1 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 4.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 290 | 20-22% PEG 4000, 200mM Li2SO4, 10% glycerol, 100mM Tris-HCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
