2IIA
Anabaena sensory rhodopsin transducer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-03 |
Spacegroup name | P 4 |
Unit cell lengths | 57.274, 57.274, 39.788 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.620 - 1.800 |
R-factor | 0.203 |
Rwork | 0.202 |
R-free | 0.23800 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.731 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.031 | 0.018 | 0.214 |
Number of reflections | 11936 | ||
<I/σ(I)> | 15.9 | ||
Completeness [%] | 98.5 | 86.8 | 92 |
Redundancy | 4.5 | 4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 100 mM sodium acetate, pH 4.6 and 7 to 11% (w/v) polyethylene glycol (PEG) 8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |