2II1
Crystal structure of Acetamidase (10172637) from Bacillus Halodurans at 1.95 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-31 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.759, 69.605, 94.984 |
| Unit cell angles | 73.95, 88.91, 86.63 |
Refinement procedure
| Resolution | 28.890 - 1.950 |
| R-factor | 0.17761 |
| Rwork | 0.175 |
| R-free | 0.22800 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.478 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.892 | 2.000 |
| High resolution limit [Å] | 1.898 | 1.950 |
| Rmerge | 0.116 | 0.681 |
| Number of reflections | 70889 | |
| <I/σ(I)> | 6.1 | 1.1 |
| Completeness [%] | 90.3 | 89.3 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20.0% POLYETHYLENE GLYCOL 3350, 0.2M CALCIUM CHLORIDE, VAPOR DIFFUSION, SITTING DROP, NANODROP, TEMPERATURE 293K |
