2IHP
Yeast inorganic pyrophosphatase with magnesium and phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8727 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.915, 93.549, 69.168 |
| Unit cell angles | 90.00, 99.66, 90.00 |
Refinement procedure
| Resolution | 19.920 - 1.500 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1huk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.445 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.041 | 0.321 |
| Number of reflections | 103964 | |
| <I/σ(I)> | 21.7 | 3.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 277 | 16% MPD, 10 mM MgCl2, 1 mM PO4-, pH 6.0, VAPOR DIFFUSION, temperature 277K |
