2IHN
Co-crystal of Bacteriophage T4 RNase H with a fork DNA substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-21 |
Detector | RIGAKU SATURN 92 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.160, 84.990, 89.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.370 - 3.000 |
R-factor | 0.225 |
Rwork | 0.222 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tfr |
RMSD bond length | 0.021 |
RMSD bond angle | 2.369 |
Data reduction software | CrystalClear |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.370 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.091 | 0.328 |
Number of reflections | 10803 | |
<I/σ(I)> | 12.7 | 3.9 |
Completeness [%] | 99.2 | 99.8 |
Redundancy | 3.81 | 3.74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 295 | 18.5% (w/v) PEG 2000 Monomethylether, 0.1M Tris-HCl, 20% (w/v) glucose, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 2000 Monomethylether | ||
2 | 1 | 1 | Tris-HCl | ||
3 | 1 | 1 | glucose | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | PEG 2000 Monomethylether | ||
6 | 1 | 2 | Tris-HCl | ||
7 | 1 | 2 | glucose | ||
8 | 1 | 2 | H2O |