2IH9
A high-dose crystal structure of a recombinant Melanocarbus albomyces laccase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.490, 62.330, 125.100 |
| Unit cell angles | 90.00, 96.25, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.263 |
| Rwork | 0.263 |
| R-free | 0.29940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ih8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 90065 | |
| <I/σ(I)> | 11.8 | 5.6 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 17% PEG MME2000, 0.1M Ammonium sulfate, 0.1M Sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
