2IGT
Crystal Structure of the SAM Dependent Methyltransferase from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-04 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 83.941, 145.555, 204.895 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.830 - 1.890 |
| R-factor | 0.16534 |
| Rwork | 0.162 |
| R-free | 0.19236 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.396 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.830 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.099 | 0.692 |
| Number of reflections | 99837 | |
| <I/σ(I)> | 11.5 | 4.3 |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 117.6 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1M sodium acetate 4.6, 3.2M sodium formate, 4% glycerol, 5mM SAM, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
