2IGQ
Human euchromatic histone methyltransferase 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.520, 95.863, 102.124 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.110 - 2.000 |
R-factor | 0.19121 |
Rwork | 0.189 |
R-free | 0.23714 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mvh |
RMSD bond length | 0.015 |
RMSD bond angle | 1.533 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.840 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.096 | 0.584 |
Number of reflections | 44160 | |
<I/σ(I)> | 8.8 | 1.3 |
Completeness [%] | 92.6 | 83.2 |
Redundancy | 3.7 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 300 | 20% PEG 4K, 10% Isoproponol, 0.1M Hepes 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |