2IGL
Crystal Structure of E. coli YEDX, a transthyretin related protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.120, 77.370, 100.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.210 - 1.800 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f86 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.066 | 0.305 |
Number of reflections | 79845 | |
<I/σ(I)> | 30 | 7.1 |
Completeness [%] | 99.3 | 97.3 |
Redundancy | 10 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 42% PEG2000 MME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |