2IGK
Crystal structure of recombinant pyranose 2-oxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-22 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.095 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 168.780, 103.860, 169.260 |
Unit cell angles | 90.00, 106.36, 90.00 |
Refinement procedure
Resolution | 39.200 - 1.800 |
R-factor | 0.17692 |
Rwork | 0.177 |
R-free | 0.21421 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.020 |
RMSD bond angle | 1.767 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.200 |
High resolution limit [Å] | 1.700 |
Number of reflections | 517862 |
<I/σ(I)> | 12.2 |
Completeness [%] | 99.0 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 12-13% monomethyl ether PEG 2000, 0.1 M mes, 50 mM CaCl2, 25% glycerol, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |