2IG8
Crystal structure of a Protein of Unknown Function PA3499 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 100.191, 100.191, 131.682 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.410 - 1.900 |
| R-factor | 0.17878 |
| Rwork | 0.178 |
| R-free | 0.19898 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.428 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.116 | 0.610 |
| Number of reflections | 50367 | |
| <I/σ(I)> | 19.1 | 1.5 |
| Completeness [%] | 99.4 | 95.5 |
| Redundancy | 9.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Tris-HCl, 3.5M Sodium formate, 4% MPD, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
