2IG3
Crystal structure of group III truncated hemoglobin from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 95.588, 95.588, 65.424 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.300 - 2.150 |
| R-factor | 0.21154 |
| Rwork | 0.206 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ux8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.940 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.067 | 0.313 |
| Number of reflections | 19090 | |
| <I/σ(I)> | 30.4 | 8.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.4 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 2.0M Ammonium sulfate, 0.1M Sodium acetate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 5.0 |
