2IFY
Structure of Bacillus anthracis cofactor-independent phosphoglucerate mutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.620, 72.737, 185.176 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.410 - 2.380 |
| R-factor | 0.265 |
| Rwork | 0.245 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.108 | 0.433 |
| Number of reflections | 22459 | |
| <I/σ(I)> | 9.9 | 2.1 |
| Completeness [%] | 91.8 | 84.2 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 24% PEG 8000. 100mM Tris-HCl, 200mM calcium acetate, 3% 1,5-diaminopentane di-HCl, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
