2IFF

STRUCTURE OF AN ANTIBODY-LYSOZYME COMPLEX: EFFECT OF A CONSERVATIVE MUTATION

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	54.800, 74.800, 79.000
Unit cell angles	90.00, 101.80, 90.00

Refinement procedure

Resolution	10.000 - 2.650
R-factor	0.183 *
RMSD bond length	0.012
RMSD bond angle	27.700 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.600 *	2.600 *
Rmerge	0.076 *
Total number of observations	37251 *
Number of reflections	12909 *	717 *
Completeness [%]	66.4 *	22.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Fab	1 (M)
2	1	1	protein	1.2 (M)
3	1	2	PEG4000	12 (%(v/v))
4	1	2	imidazole	0.1 (M)
5	1	2	spermine	8 (mM)