2IF5

Structure of the POZ domain of human LRF, a master regulator of oncogenesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	I 41 2 2
Unit cell lengths	65.850, 65.850, 162.603
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.080 - 2.000
R-factor	0.23245
Rwork	0.229
R-free	0.29096
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1buo
RMSD bond length	0.017
RMSD bond angle	1.580
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.130
High resolution limit [Å]	2.000	2.030
Rmerge	0.096	0.259
Number of reflections	12540
<I/σ(I)>	25.9	9
Completeness [%]	98.2	99.8
Redundancy	13	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	290	Protein at 24 mg/ml in 25 mM Tris, 150 mM NaCl, 2mM DTT, 0.18 M Calcium acetate hydrate, 18% w/v Polyethylene glycol 3350, 0.01 M Praseodymium (III) Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K