2IEQ
Core Structure of S2 from the Human Coronavirus NL63 Spike Glycoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-04-04 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.020, 51.688, 54.080 |
Unit cell angles | 90.00, 107.56, 90.00 |
Refinement procedure
Resolution | 51.570 - 1.747 |
R-factor | 0.19866 |
Rwork | 0.196 |
R-free | 0.23977 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zv8 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.502 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.570 | 1.810 |
High resolution limit [Å] | 1.747 | 1.747 |
Rmerge | 0.068 | 0.363 |
Number of reflections | 25668 | |
<I/σ(I)> | 10.7 | 3.7 |
Completeness [%] | 95.6 | 98.1 |
Redundancy | 3.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | PEG 400, sodium acetate, Imidazole, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |