2IEP
Crystal structure of immunoglobulin-like domains 1 and 2 of the receptor tyrosine kinase MuSK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.92014 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.608, 118.004, 57.785 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.205 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fhg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.205 | 2.205 |
| Rmerge | 0.060 | 0.331 |
| Number of reflections | 27127 | |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 98.7 | 97.5 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 8% PEG 4000, 0.2M Ammonium Sulfate, 0.1M Sodium Acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
