2IEK
New crystal form of transcriptional regulator tm1030 from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.566, 52.286, 49.665 |
| Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
| Resolution | 19.870 - 1.830 |
| R-factor | 0.22159 |
| Rwork | 0.219 |
| R-free | 0.26915 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zkg |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.504 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.128 | 0.437 |
| Number of reflections | 17541 | |
| <I/σ(I)> | 28.8 | 4.3 |
| Completeness [%] | 98.3 | 100 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.05M Na citrate, 30% w/v PEG 2K MME, 0.1M KSCN, 0.01M NH4 sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
