2IEF
Structure of the cooperative Excisionase (Xis)-DNA complex reveals a micronucleoprotein filament
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 111.655, 111.655, 76.268 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.810 - 2.601 |
| R-factor | 0.20105 |
| Rwork | 0.199 |
| R-free | 0.24577 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.653 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.250 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 16295 | |
| <I/σ(I)> | 24.4 | 5.13 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 30% PEG 4000, 0.2M Ammonium Acetate 0.1M Sodium Citrate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | Ammonium Acetate | ||
| 3 | 1 | 1 | Sodium Citrate | ||
| 4 | 1 | 2 | PEG 4000 | ||
| 5 | 1 | 2 | Ammonium Acetate | ||
| 6 | 1 | 2 | Sodium Citrate |
