2IEE
Crystal Structure of YCKB_BACSU from Bacillus subtilis. Northeast Structural Genomics Consortium target SR574.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97916, 0.97943, 0.96791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.284, 49.918, 106.768 |
| Unit cell angles | 90.00, 99.73, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.200 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.24100 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.065 | 0.198 |
| Number of reflections | 50187 | |
| <I/σ(I)> | 17.2 | 6.8 |
| Completeness [%] | 88.3 | 85.95 |
| Redundancy | 17.2 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 34% PEG 1000, 100 mM TAPS, 100 mM Ammonium nitrate, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
