2IE2
The 1.7 A crystal structure of Dronpa: a photoswitchable green fluorescent protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 103.140, 175.499, 67.441 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.090 - 1.700 |
| R-factor | 0.19922 |
| Rwork | 0.198 |
| R-free | 0.22093 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xss |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.945 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.090 | 1.748 |
| High resolution limit [Å] | 1.700 | 1.704 |
| Rmerge | 0.085 | 0.573 |
| Number of reflections | 129416 | |
| <I/σ(I)> | 22.8 | 2.4 |
| Completeness [%] | 96.3 | 95.6 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 23% PEG 3350, 0.1M Tris, 0.1M Magnesium Chloride, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
